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| tag | N/A |
| host species | Human |
| accession | P01137 |
| synonym | TGFβ-1 Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB |
| background | Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone, articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGFβ-1 performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide. TGFβ-1 may also form heterodimers with other TGFβ family members. It has been found that TGFβ-1 is frequently upregulated in tumor cells. Mutations in this gene results in Camurati-Engelmann disease. |
| description | Recombinant Human Transforming Growth Factor beta 1 / TGFβ-1 is expressed by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed. |
| source | CHO Stable Cells |
| AA sequence | Ala279-Ser390 |
| molecular weight | 12.8kDa AP Mol Mass 13kDa, reducing conditions. |
| purity | >95% as determined by reducing SDS-PAGE. |
| endotoxin | Less than 0.01 EU/µg of human TGFB1 protein as determined by LAL test. |
| bioactivity | Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 mouse T cells. The ED50 for this effect is 4-40 pg/ml |
| formulation | Recombinant Human TGFβ-1 / tgfb1 Protein was lyophilized from a 0.2 μm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH 2.5. |
| stability | Recombinant Human TGF bera 1 proteinis are stable for up to 12 months at -80℃ |
| reconstitution | Dissolve the lyophilized protein in distilled water. It is not recom-mended to reconstitute to a concentration less than 100μg/ml |
| usage | For Research Use Only |
| storage | Human TGFβ-1 protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
