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DNA Polymerase I, Large (Klenow) Fragment

Cat No.: ENY-104
description DNA Polymerase I, Large (Klenow) Fragment (about 68 kD) is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3'→5' exonuclease activity, but has lost 5'→3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini. It is applicable to DNA sequencing by the Sanger dideoxy method, fill-in of 5´ overhangs to form blunt ends, removal of 3´ overhangs to form blunt ends, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols. Theoretical protein molecular weight is 68000 daltons. Error Rate: ~ 18x10-6 bases
source An E.coli strain that contains the E. coli polA gene that has had its 5'→3' exonuclease domain removed.
applications Blunting, DNA Sequencing , Polymerases for DNA Manipulation, RT-qPCR, RT-PCR and cDNA Synthesis, RT-PCR & cDNA Synthesis, PCR
size 200 U / 1000 U / 5000 U
concentration 5,000 U/ml
components DNA Polymerase I, Large (Klenow) Fragment (5,000 U/ml); 10X ABuffer B
unit definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.
usage For Research Use Only
storage Storage condition: 25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @25℃. Store the DNA Polymerase I, Large (Klenow) Fragment at -20°C. Please avoid repeated freeze-thaw cycles.
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